Adjuvant effect of cholera toxin on systemic and mucosal immune responses in chickens infected with E. tenella or given recombinant parasitic antigen per os.

نویسندگان

  • F Girard
  • P Péry
  • M Naciri
  • P Quéré
چکیده

We investigated the adjuvant effect of cholera toxin (CT) on the intestinal and systemic immune systems of chickens. The CT was given orally, mixed with a non-replicating antigen, a recombinant Eimeria protein, IPEI, or with a replicating one, Eimeria tenella parasite. There were increases in the specific IgA and IgG responses to the recombinant protein IPEI, with a significant anti-1PE1 IgG response in the duodenum (p < 0.05) and caecum (p < 0.05) 4 weeks after immunization and a specific IgA (p < 0.05) response in the duodenum after 3 weeks. A transient anti-1PE1 IgG (p < 0.05) response was detected in the serum 1 week post-injection and an IgA response (p < 0.05) at 2 weeks. CT given with the replicative parasite caused no change in the intestinal and systemic immune responses with 1 or 3 immunizations although a specific antiparasitic in vitro proliferation of the spleen cells from infected chickens was observed. Nevertheless, 0.05 mg CT given per os to chickens was strongly immunogenic in both experiments. A strong serum IgG (p < 0.01) response was detected as soon as 1 week after the end of the immunization protocol with 1PE1 and 2 weeks after infection with E. tenella. Strong anti-CT IgG responses were also detected by the second week post-immunization in the duodenum and caeca (p < 0.01). Hence, CT can be used as a mucosal adjuvant in chickens to improve the intestinal immune response.

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عنوان ژورنال:
  • Vaccine

دوره 17 11-12  شماره 

صفحات  -

تاریخ انتشار 1999